Using Human Cancer Cell Lines as In vitro Model for Testing the Efficacy of CDBPA

The use of tissue culture methods in oncology has been performed for the last many decades, where Beebe and Ewing tried to grow lymphosarcoma in dogs. An increasing variety of in vivo tests are used in cancer research to detect the optimal therapy and to study cancer cell functions. in vivo experiments indicate that these in vivo techniques are oftenthe leading techniques initially when a potential anticancer drug is going tobe tested for its ability to kill cancer cells. Improved cell culture techniques and application of molecular biology and genetic techniques will be more and more useful in the efficacy studies of anti-cancer drugs research as recommended by the European Community (Europe against cancer). 

However, we agree with Weaseling and Draws, that in vivo tests can`t abolish in vivo studies in testing any cancer agents nowadays. But we feel that in vivo testing can reduce the number of painful animal experiments to be reduced substantially. This together with amethodology approach created to reduce the number of animals for research is animportant issue. We have used in vivo studies to determine the potential of the new innovative anti-cancer substance CDBPA (BP-C1) cis-coordinated complexes of platinum (II) with polymer of benzene poly-carboxylic acids derived from lignin, on the viability of human breast cancer cells.